Hello All,

We are trying to refine a complex that was solved using Phaser.  We have run a round of rigid body refinement using Refmac and now want to run a simulated annealing run in Phenix.  Visually the complex looks pretty.  The resolution we currently are at is 3.6 A.  When we go to run phenix.refine the program quits with a Number of non-bonded interactions < 0.0001 error.  Is there a way to loosen this up a bit or get around it or should we go about this in a different way?  

Best Regard,

Leonard Thomas, Ph.D.
Macromolecular Crystallography Laboratory Manager
University of Oklahoma
Department of Chemistry and Biochemistry
Stephenson Life Sciences Research Center
101 Stephenson Parkway
Norman, OK 73019-5251
Office: (405)325-1126