Dear Tom <br> <br>Thankyou for your kind reply.I checked my data witn phenix.xtriage<br>and there are good anomalous signal (&gt;0.05)in all the resolution shells.<br>Also the systematic absences show it is P43212.Still i couldnt<br>
solve using phenix.Where is the thing going wrong?<br>Xtriage results also showed no twinning.<br><br><br>My previous message:<br>I am using phenix1.4-4 version. In autosol, got the score of 10.24 for one iodine soaked data.<br>

FOM:0.27 and only 54 residues ( that too wrongly) were built out of 129.(CC=0.4), R=0.49 and Rfree=0.574.<br>

Regrading the data quality, Rsym:0.0448, Ranom:0.055, Resln:25.0-2.6A<br>
Spacegroup is P43212, completeness:99.4%, Multiplicity:11.7, anomalos<br>
signal is 1.52 at lowest resoln and higher in high resolution shells.<br>
<br>

What might be reason for not  able to solve this structure?<br><br><br>