Re: [phenixbb] Q. Phase extension

Hi Nat, I suggest that the phase extension plus the availability of experimental phases at low resolution is key here. The poor model will get refined (yes, not very good phases there), combined with experimental phases (still a bit weak), but then extended gradually to high resolution (possibly greatly improving the phases), and then model-building is done. I think it could work. All the best, Tom T On Jun 26, 2013, at 9:20 AM, Nathaniel Echols wrote:

On Wed, Jun 26, 2013 at 2:05 AM, Mark J van Raaij wrote:

...

Can't you just refine the poly-Ala model against the 1.5A native data? (Perhaps after rigid body refinement or even molecular replacement if the data is too non-isomorphous). And then run Autobuild with these phases?

If the model only contains around 50% of residues, and no sidechains, the phases from refinement are going to be fairly poor. I've never had much luck trying to autobuild starting such low completeness, even with very good data.

-Nat _______________________________________________ phenixbb mailing list [email protected] http://phenix-online.org/mailman/listinfo/phenixbb