Dear All,

After check my data with Phenix Xtriage, I got a warning of "the anomalous completeness is 42.34%". 

I do not know if I need to pay attention to this? I did not do experimental phasing and I did not add heavy metal to my crystal nor there is selenium on my protein. I even don't know why I still get the anomalous signal.  

But from the detailed summary below I guess in my case it's not a problem?


                          ----------Summary----------

                              File name:                        P2_rejoutput.sca
                            Data labels:               I(+),SIGI(+),I(-),SIGI(-)
                            Space group:                                 P 1 2 1
                              Unit cell: 72.255, 46.617, 134.086, 90, 90.052, 90
                              Data type:                          xray.intensity
                             Resolution:                        49.1682 - 2.5855
                              Anomalous:                                    True
  Number of reflections (non-anomalous):                                   27184
           Completeness (non-anomalous):                                  95.42%
            Number of reflections (all):                                   38285
                     Completeness (all):                                  69.98%
                 Anomalous completeness:                                  42.34%

  Completeness (non-anomalous) is the completeness of the data after merging
  Friedel pairs.

  Completeness (all) is the completeness of the data before merging Friedel
  pairs.

  Completeness (anomalous) is the completeness of the anomalous data. The
  anomalous completeness is calcluated by dividing the number of measured
  acentric, Bijvoet mates by the total possible number of acentric indices.

  Completeness (non-anomalous) should be used to determine if there is
  sufficient data for non-anomalous purposes (refinement, model-building).
  A value greater than 90% is generally desired, while a value less than
  75% is considered poor. Values in between will provide less than optimal
  results.

  Completeness (anomalous) should be used to determine if there is sufficient
  data for anomalous purposes (phasing, finding anomalous atoms, refinement
  of anomalous occupancies or scattering factors). A value greater than 80%
  is generally desired, while a value less than 50% is considered poor. Values
  in between will provide less than optimal results.

Thanks.