Hello Chris,
The standard question here is: Are you certain of your space group? That seems to be the main culprit behind R factors that wont decrease.
Can you provide some statistics showing your confidence in your space group?

Kelly Daughtry

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Kelly Daughtry, Ph.D.
Post-Doctoral Fellow, Raetz Lab
Biochemistry Department
Duke University
Alex H. Sands, Jr. Building
303 Research Drive
RM 250
Durham, NC 27710
P: 919-684-5178
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On Wed, Jan 12, 2011 at 5:12 PM, Christopher W Davies <cdavies@purdue.edu> wrote:


I am having an issue with refining my structure. 狢 have a 2.36 A resolution dataset. 狢 have found a solution by molecular replacement in the space group P3221. 狢 have been refining the structure, however, my Rfactor/Rfree are not decreasing even though I have good density. 瓱y current Rfactor/Rfree values are .40/.43. 蟖an anyone help me?


Thanks.

Chris
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