Re: [phenixbb] few questions about resolving new structure through MR

On Thu, Nov 7, 2013 at 10:44 AM, Zhihong Yu wrote:

Thanks for your reply. So from your answer, I feel that maybe it's not worth to spend much time to resolve the structure through MR based on current dataset? Even I can correctly place the domainB, it's still hard to resolve the intact protein structure without other phasing information, is that right?

There's no absolute rule, but it's extremely difficult even with higher resolution data. Sometimes density modification would help but I suspect the phases just won't be good enough. With an R-free of 0.55 it is very unlikely that you'll be able to see anything interpretable in the maps, even if the model placement is correct. -Nat