26 Jul
2016
26 Jul
'16
4:38 p.m.
Hi Jason, could you please send me PDB file or fraction of it containing the brake in question? Pavel On 7/26/16 07:54, Booe, Jason M (HSC) wrote:
Dear all,
I am refining a structure after renumbering my chain such that there is a gap in numbering (i.e. chain A ends at 350 and then starts again at 1000) as this has numbering has been recommended for us to use by the PDB for our fusion proteins. I'm having issues with Phenix.refine not understanding that their is still a peptide bond and breaks the chain and pushes the atoms away. There is no TER card between the chains and I've used LINK records as well to no avail. I'm wondering how to properly deal with this.
Thanks, Jason