Hi Dmitry,
I am guessing that your structure contains C2 symmetry as well as helical symmetry, is that correct? The find_ncs algorithm can do one or the other, but not both at once.  For your case I think you want to specify:

    reconstruction_symmetry.symmetry=helical
    include_helical_symmetryTrue

and it should look for helical symmetry only.
All the best,
Tom T


On Sun, Oct 22, 2023 at 11:20 AM Dmitry Semchonok <semchonok@gmail.com> wrote:

Dear colleagues,

 

Please prompt me on correctly applying the symmetry parameters to the helical cryo-em map.

 

What I did:

  1. Found symmetry with Map symmetry. The process finished successfully.
  2. Applied the NFC operators.  

But the outcome has only two units (C2 symmetry).
When I put more units in the program setting, nothing happens after the run; moreover, the output is not generated.

 

 Could you please advise how to fill the helical cryo-EM structure with units?

 
Many thanks for considering my request.

 

Sincerely,

Dmitry


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--
Thomas C Terwilliger
Laboratory Fellow, Los Alamos National Laboratory
Senior Scientist, New Mexico Consortium
100 Entrada Dr, Los Alamos, NM 87544
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