Hi everyone,<br>I have came into a problem recently. My structure was solved by MAD and a crude model was built manually.<br>Several peak data sets were collected, resolution is about 4A-4.2A. The heavy atom can be located by anomalous difference fourier. I feed the initial phase or heavy atom sites, and the peak data set to phenix. However the map after DM is too bad. The density is very strange. I don't know what's the problem?<br><br>In addition, how to define protein mask at phenix autosol for density modification?<br><br>Thanks in advance.<br><br><br><div>--<br><br><font fac="Arial, Verdana" color="#127db8">Yan Zhao, M.Phil.<br>National Laboratory of Protein Sciences <br>Institute of Biophysics <br>Chinese Academy of Sciences <br>15 Datun Rd.<br>Beijing, 100101 <br>China </font></div>