Actually, I think I found how to override the iso/aniso decision: under "global parameters," set aniso cutoff to lower resolution. Seems to be working. Any thoughts on the more general question about aniso refinement in the case of truncated data, or +/- twinning? Seems twinning changes ML refinement to LSQ, but I am not sure how that changes this. Jacob -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Keller, Jacob Sent: Friday, July 31, 2015 3:53 PM To: [email protected] Subject: [phenixbb] Anisotropic B-factors with Trucated Data Dear Crystallographers, I have a dataset which was artificially truncated at 1.7 Ang by the limitations of our setup, but the resolution is probably 1.5 Ang or so. I would like to try running anisotropic B refinement in Phenix, but am not sure how to tweak to get this to happen. I know about the parameter:measurement ratio concept, but I think it may still be better than isotropic. NB the dataset is also 42% twinned. All the best, Jacob Keller ******************************************* Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: [email protected] ******************************************* _______________________________________________ phenixbb mailing list [email protected] http://phenix-online.org/mailman/listinfo/phenixbb Unsubscribe: [email protected]