Hi Jason, - run your model through TLSMD server to identify TLS domains (it will produce PHENIX friendly TLS groups selections); http://skuld.bmsc.washington.edu/~tlsmd/ - use these selections for TLS refinement in PHENIX: http://www.phenix-online.org/documentation/refinement.htm for example, the command is: phenix.refine model.pdb data.hkl strategy=individual_sites+individual_adp+tls tls_selections.txt Pavel. On 10/17/2008 3:10 AM, [email protected] wrote:

Hi All,

I just refined a structure at 2.95A and getting a good R-work and R-free(0.236 and 0.274). When I checked the PDB, I found many residues have high temperature factor over 100. How can I use Phenix or CNS fix those high temperature factor for further refinment?

Thank you Jason

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