Hi Mohamed, I solved a structure at 1.5 A with Sulfur MAD. There were only 4 Sulfurs in the sequence and the signal has very nice. Pay attention S does not give high anomalous signal. I suggest you collect a dataset at 7kev (around 2.0A) which is the absorption edge of S. You must collect very high multiplicity around 30-40 times with low intensity beam not to burn the crystal. This at kappa 0 and kappa 45. Then you could merge both datasets. I remember an important thing, on the absorption edge we had to be careful on the harmonics and make a nice setting on the beam to remove them. I suggest you do also a collection at 14kev far away from absorption edge. For my case only Autorickshaw found the Sulfurs and solve the problem of phases. Hope it helps, Ivan Sent with AquaMail for Android http://www.aqua-mail.com On 27 July 2015 7:17:18 pm mohamed noor wrote:

Dear all

Would you have some tips on collecting datasets for S-SAD at a 'normal' beamline (tunable from 0.5-2.5 A)? How helpful/important is it to use mini kappa?

Thanks.

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