Hi ??, hm, this shouldn't normally happen! Could you please send me relevant inputs so I can reproduce the problem and see what exactly is happening? Also explain how you run autobuild and then phenix.refine. Pavel On 7/20/12 5:43 AM, ?? wrote:

Hi all: I do not know if someone mentioned this problem here before. Recently I ran the AutoBuild to build the structure model, and it outputs a best model(R/Rfree 0.20/0.24). After that, I tried the program Phenix Refine using the .pdb and .mtz generated by AutoBuild. The R value increased to 0.23/0.30 by the end of this refine. I wonder why this happened? Or if I have done something wrong?