Hi John, my understanding is that SFCHECK does not reproduce the TLS or bulk solvent scaling. Depending on your structure this can be a huge effect on the R-factors. Cheers, Paul On Sep 22, 2008, at 8:35 PM, john kryst wrote:

Hi all, I have a little problem... R and R-free from phenix.refine are 22.9 and 30.6 respectively.... when i validated in the rcsb server... sfcheck gave me a R and Rfree of 30.8 and 37.8. I am aware that sfcheck never reproduces the result. but i am worried that the difference is quite large... indeed i have used TLS NCS and bulk solvent correction...

Did i do anything wrong... please help me

Thanks for your help

regards John _______________________________________________ phenixbb mailing list [email protected] http://www.phenix-online.org/mailman/listinfo/phenixbb

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