Hi Francis, Yes, you can do that. It requires one extra step to do the combination. You need to make a script like the one below (see https://solve.lanl.gov/Resolve/html_resolve_manual/resolve_sample_scripts.ht...): All the best, Tom T Merging phase sets with different origins You can merge phase sets with different origins with this script. It uses an FFT convolution to find the best allowed offset between the maps calculated with the two phase sets. Merging the files REQUIRES HL coefficients (see above for generating them if you do not have them). Note: the FP and SIGFP in the output file will come from the hklin file NOT the hklmerge file: phenix.resolve <

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Thanks so much!It looks promising. If I have two or more derivative data and want to combine all these information. Can I just do MRSAD individually for each dataset, and combine them?

ÒýÓà Nathaniel Echols £º

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On Tue, Jun 15, 2010 at 4:03 PM, Francis E Reyes

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Using the model to find the heavy atom sites and then calculating phases on those sites (without model) might help. ( I believe phenix will output a phase combined map (model + experimental phases)).

Correct, this is the default behavior if you provide a model directly to AutoSol (or Phaser). There is however a semi-automatic way to use the model phases to find sites without biasing the experimental map; see the documentation here:

https://www.phenix-online.org/version_docs/1.6.2-432/autosol.htm#anch126

(The AutoBuild step is optional - you could just look at the density-modified map after AutoSol is done.)

-Nat

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