Hi Francis, You will still have restraints on the backbone, you just won't be able to use rotamer outlier correction, which is what those errors are reporting. If you want to silence those errors, you can turn outlier correction in the options. Jeff On Tue, Nov 19, 2013 at 7:03 PM, Francis Reyes wrote:

I'm refining a structure ( at low res ), so I trimmed off all the side chain atoms from my model while my reference model still has these chains. I'm receiving errors like... ----------Correcting rotamer outliers to match reference model----------

** evaluating rotamers for working model ** A 502 ILE is missing some sidechain atoms, **skipping** A 504 LYS is missing some sidechain atoms, **skipping** A 505 VAL is missing some sidechain atoms, **skipping**

I don't care about the side chain atoms, but I'd still like the reference model restraints to provide secondary structure. Does "**skipping**" mean that the reference model is no longer being considered for the particular residue? Or will I still retain my secondary structure from the reference model?

Thanks,

F

--------------------------------------------- Francis E. Reyes PhD 215 UCB University of Colorado at Boulder

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