On Fri, Sep 21, 2012 at 3:03 AM, Mikalai Lapkouski wrote:

I have a following question. I have generated several Fo-Fc sa omit maps (FOFCWT, PHFOFCWT) for different portions of a DNA molecule. Now that I have each omit map as a separate file to display the whole DNA omit map I have to load them in coot one by one. Is there a way of how to combine all this maps into one file and have a single name for label columns so that the whole map will open at once?

I managed to put them all in one file but column labels are separate: FOFCWT_1, PHFOFCWT_1 FOFCWT_2, PHFOFCWT_2...... Can they all go under FOFCWT, PHFOFCWT?

No, one of the limitations of the MTZ format is that column labels must be unique. -Nat

Not a trivial undertaking, but it can be done. After all, CNS composite omit map does output (optionally) the map coefficients. The way it is done there is by doing fourier transform on the output map. So, you can do something along these lines 1. Calculate electron density maps from each of your truncated models. 2. Generate masks for each of your truncated models on the same grid. 3. Combine maps with corresponding masks. 4. Combine all the resulting masked maps. 5. Calculate structure factors from the resulting composite map. There are definitely programs out there (sfall, mapman, etc) that you can use top accomplish this. This would be a great learning experience for you, however if you are more of a practical person and simply want to get that final map asap, you are probably better off rerunning your composite omit map calculation using one of the following tools http://www.phenix-online.org/documentation/autobuild.htm#anch174 http://cns-online.org/cgi-bin/cns_solve_1.3/cns_view.cgi?&file=inputs/xtal_refine/composite_omit_map.inp http://www.ccp4.ac.uk/ccp4/html/comit.html Cheers, Ed. On 09/21/2012 06:03 AM, Mikalai Lapkouski wrote:

Hello,

I have a following question. I have generated several Fo-Fc sa omit maps (FOFCWT, PHFOFCWT) for different portions of a DNA molecule. Now that I have each omit map as a separate file to display the whole DNA omit map I have to load them in coot one by one. Is there a way of how to combine all this maps into one file and have a single name for label columns so that the whole map will open at once?

I managed to put them all in one file but column labels are separate: FOFCWT_1, PHFOFCWT_1 FOFCWT_2, PHFOFCWT_2...... Can they all go under FOFCWT, PHFOFCWT?

Thank you very much for any suggestion

Mikalai

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