Re: [phenixbb] poor phasing
Dear members,
I am using phenix1.4-4 version. In autosol, got the score of 10.24 for one iodine soaked data. FOM:0.27 and only 54 residues ( that too wrongly) were built out of 129.(CC=0.4), R=0.49 and Rfree=0.574. Regrading the data quality, Rsym:0.0448, Ranom:0.055, Resln:25.0-2.6A Spacegroup is P43212, completeness:99.4%, Multiplicity:11.7, anomalos signal is 1.52 at lowest resoln and higher in high resolution shells.
What might be reason for not able to solve this structure?
Hi Vennila, I would suggest running phenix.xtriage on your data, and examining in particular its analysis of 1. twinning 2. possible space groups (are your certain that there is a screw axis?) 3. measureability of anomalous signal as a function of resolution In 1.4-4 you may have to run both p43212 and p41212 (current versions do this for you). All the best, Tom T
Dear members,
I am using phenix1.4-4 version. In autosol, got the score of 10.24 for one iodine soaked data. FOM:0.27 and only 54 residues ( that too wrongly) were built out of 129.(CC=0.4), R=0.49 and Rfree=0.574. Regrading the data quality, Rsym:0.0448, Ranom:0.055, Resln:25.0-2.6A Spacegroup is P43212, completeness:99.4%, Multiplicity:11.7, anomalos signal is 1.52 at lowest resoln and higher in high resolution shells.
What might be reason for not able to solve this structure?
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On Apr 24, 2009, at 2:31 AM, vennila Natesan wrote:
I am using phenix1.4-4 version.
FYI, everyone* should be using either the current 'stable' release (1.4-3) or a recent nightly build. There were a few major bugs in 1.4-4 that are now fixed and it was the last installer before we switched to the latest version of Phaser - I'm not sure why so many people ended up using it. -Nat (* Unless there's a specific reason to use 1.3-final, such as backwards compatibility with older configuration files.) ------------------- Nathaniel Echols Lawrence Berkeley Lab 510-486-5136 [email protected]
participants (3)
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Nathaniel Echols -
Thomas C. Terwilliger -
vennila Natesan