Almudena The restraints applied to RNA is based on the starting puckers. It tries to restrain it to the 2-prime or 3-prime starting geometry. Cheers Nigel --- Nigel W. Moriarty Building 64R0246B, Physical Biosciences Division Lawrence Berkeley National Laboratory Berkeley, CA 94720-8235 Phone : 510-486-5709 Email : [email protected] Fax : 510-486-5909 Web : CCI.LBL.gov On Mon, Apr 13, 2015 at 8:34 AM, Almudena Ponce Salvatierra < [email protected]> wrote:

Dear all,

does anyone know how phenix.refine deals with the sugar puckering of an RNA or a DNA strand? Does it refine it? Or does it leave it as it was built?

Thank you very much in advance.

Best wishes,

Almudena

-- Almudena Ponce-Salvatierra Macromolecular crystallography and Nucleic acid chemistry Max Planck Institute for Biophysical Chemistry Am Fassberg 11 37077 Göttingen Germany

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