Hi Bishal - I notice you have something funny happening in the 3.8 - 3.3 A bin as well. Did you have ice ring problems in your data? I suspect your funny R factors are coming from outliers in your reflection data. Reprocessing the data with a careful eye to scaling problems or ice rings may help you here. If this doesn't help, you may need to collect a new dataset... Good luck, Matt On 2/25/15 1:28 PM, Singh, Bishal wrote:

Hello everyone, I am refining a structure at 2.3 A resolution. R-work/R-free is 26/31 and is not reducing further. When I looked into the resolution bin, I notices a sharp rise of R-free between resolution range 2.5-2.6 A (marked in figure below). However R-work is fine. I am doubtful of reducing the resolution to 2.5 A or lower. Please suggest me your opinion. Your words will be highly appreciated.

With regards, Bishal Singh PhD Student University of Heidelberg, Germany

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