FSC curve between experimental cryo EM map and model
Dear all, If I want to calculate FSC curve between experimental cryo EM map and model, what is the correct procedure to do so? I will explain what I have tried so far. Although I have variable local resolution in my map, according to FSC=0.143 criterion I have a 4 Ang map (say 1.mrc), I have used 'molmap' command in Chimera to generate a simulated map at 4 Ang. Then I used "vop resample" to take care of the pixel and box values ( then saved as 2.mrc). Thereafter, I have used "e2proc3d.py 1.mrc fsc.txt --calcfsc=2.mrc and plotted the fsc.txt file to get the curve. I am not sure if I am on the right track. My pixel size is 1.28. Best regards, Tofayel
Hi Tofayel, phenix.mtriage (analogue of phenix.xtriage) is likely to be available some time next week in one of Phenix nightly builds. It will report various FSC. Pavel On 4/28/17 06:08, #AHMED TOFAYEL# wrote:
Dear all,
If I want to calculate FSC curve between experimental cryo EM map and model, what is the correct procedure to do so?
I will explain what I have tried so far. Although I have variable local resolution in my map, according to FSC=0.143 criterion I have a 4 Ang map (say 1.mrc), I have used 'molmap' command in Chimera to generate a simulated map at 4 Ang. Then I used "vop resample" to take care of the pixel and box values ( then saved as 2.mrc). Thereafter, I have used "e2proc3d.py 1.mrc fsc.txt --calcfsc=2.mrc and plotted the fsc.txt file to get the curve.
I am not sure if I am on the right track. My pixel size is 1.28.
Best regards,
Tofayel
participants (2)
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#AHMED TOFAYEL# -
Pavel Afonine