Hi Dialing, run the same refinement with weights optimization. You can make it faster by specifying a number of CPUs that phenix.refine can use. Pavel On 1/31/15 7:30 AM, Dialing Pretty wrote:

Dear All, After a Phenix refinement (for crystal 2.5A), my Rwork is about 0.19, and my Rfree is about 0.26. Is the gap between Rwork and Rfree too karge? If too large, why it is too large and how to avoid it? I am looking forward to getting your reply. Dialing