Ramachandran plot restraints are rather to preserve good conformations from becoming outliers (in cases there is no data to support it or data is insufficient) than fix existing outliers. Pavel On 2/24/15 5:58 PM, Smith Lee wrote:

Dear All,

If my resolution was between 2.5-3.0 A, and if I did not use Ramachandran restraints in the Phenix refine the Ramachandran outliers would be increased significantly during the refinement process, in this situation may I use Ramachandran restraints in the Phenix refine?

I am looking forward to getting your reply.

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