here is my suggestion: mosflm data needs to be scaled (use scala in ccp4) (make sure anomalous is turned on) (this should give you a mtz file with anomalous data) then in the phenix gui under wizards, choose AutoSol the first screen will ask you to choose your mtz and experiment type (sad) the next screen will ask the file name again, but will be already filled in, leave it the next screen will ask what columns to output, you can remove all but the anomalous I's (either the +/- or the DANO/SIGDANO format) (will be written as a sca file after this step) the next screen will look like the first, and should just have a single sca file on it (output from the previous screen) the next few screens allow you to set the experiment parameters if all goes well, auto sol should start running after 3 or 4 more screens On Tue, 11 Jul 2006 [email protected] wrote:

Hi all i am using the Phenix-GUI first time ,for solving the sad data. I processed my raw data with the mosflm.Further output of the mosflm were used for getting other information or working through this data.so, i started with the strteiges of solve through mad. after setinmg up all the anamolous parameters ,this is always displaying red in the very first in the setup anamolous data gui with the information that format error.i used the format ccp4 unmerged i.e the direct output of the mosflm after integration.i also used the conversion of mtz file into hkl through ccp4 scaling and merging intensities-in which one option i used is the get output as unmerged intensities but, that also didnot worked. suggestions will be appreciated and also i want the deatiled information about how to use the phenix gui properly. with regards

Pramod Kumar Junior Research Fellow c/o-Dr.Shekher C Mande Center for DNA Fingerprinting and Diognostics ECIL road Nacharam Hyderabad-76

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