on real_space_refine
Dear All, After fitting a PDB into a 3.5-5 A resolution EM map by phenix.real_space_refine, should I still need to do a reciprocal space refine? For crystallography, the reciprocal space is in fact not purely imaginary, it corresponds to the diffaction spot. But for the EM data refinement, what is the reciprocal space refine? And what reciporce spce refine works on for the data refinement? Smith
After fitting a PDB into a 3.5-5 A resolution EM map by phenix.real_space_refine, should I still need to do a reciprocal space refine?
Why?
For crystallography, the reciprocal space is in fact not purely imaginary,
Indeed!
But for the EM data refinement, what is the reciprocal space refine?
If you imagine cryo-EM data to be in reciprocal space you can do reciprocal space refinement then. This may work given you know why you do this. Pavel
participants (2)
-
Pavel Afonine -
Smith Liu