Hi Alex, On the TaBr cluster: I'm sorry as you noticed this was broken in hyss and autosol. It was just fixed a week ago...however we have not had any builds released since then. Once the next nightly build is released you should be able to use "TX" as your atom type in either hyss or autosol. All the best, Tom T ________________________________ From: [email protected] [[email protected]] on behalf of Alexander Kintzer [[email protected]] Sent: Saturday, August 30, 2014 12:08 PM To: [email protected] Subject: [phenixbb] Phasing using CryoEM Map Hello All, I trying to phase my 4Å data with low resolution cryoEM maps (various 12-18Å). There is some documentation, but not really for dealing with EM data. I am having trouble converting the EM data from mrc to suitable F's for phaser. One would want to try different density contours of the cryoEM map, but I haven't found a good way to accomplish this. I also have been unable to search for Ta6Br12 clusters using Autosol even though the documentation seems to suggest that it is possible using the "TX" or "XX" designation for heavy atom search. Any guidance would be extremely helpful. best, Alex

Hi Alex,

I trying to phase my 4Å data with low resolution cryoEM maps (various 12-18Å). There is some documentation, but not really for dealing with EM data. I am having trouble converting the EM data from mrc to suitable F's for phaser.

isn't it a matter of Fourier transforming your map into structure factors and then using them down the road pretending that these are your "Fobs" and however obtained phases? If that's the case then phenix.map_to_structure_factors is the tool you need: http://www.phenix-online.org/documentation/reference/map_to_structure_factor... If not I wonder what am I missing? Pavel