Dear Smith, the model building menu in coot has an "Add Alt Conf..." entry. Click it, and you get what you want. Best, Tim On Monday, September 07, 2015 09:31:24 PM Smith Liu wrote:

Dear All,

In my map for a specific HISresidue, it was rather clear the sidechain has 2 conformations. In my start modle, I only have the PDB for 1 of the 2 conformations of the sidechain. With "occupancies" clicked, the refined PDB still only contain the PDB for 1of the 2 conformations of the sidechain (occupancy is 1).

Thus in order to have the GUI "occupancies" takes function, I need manually add the PDB for the conformation of the other side chain, am I right? If I am right，will you please show me an PDB example which contains the conformations for 2 or several sidechains of a residue, so that I can know how to edit this kind of PDB?

Best regards.

Smith

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