Postdoctoral position in cryo-EM and translation regulation ‒ from bacteria to neurons Korostelev Lab, University of Massachusetts Medical School We study how the ribosome interacts with numerous cellular components to achieve high accuracy and efficiency of gene expression. We’d like to understand the key roles of translation and ribosomes in: (1) Neurodevelopment and neurodegenerative diseases; (2) Bacterial stress signaling and gene regulation (e.g. coupling with transcription); and other aspects of gene expression (https://www.umassmed.edu/korostelev-lab/). We use a range of approaches, including cryo-EM and biochemistry (see selected references below). Our laboratory is housed at the RNA Therapeutics Institute, a vibrant and highly interactive research community: https://www.umassmed.edu/rti/about-the-rti/faculty-members/. Our colleagues are interested in nearly all aspects of RNA biology, from small to large RNAs to RNA chemistry and therapeutics development. We seek a highly motivated person who very recently received a PhD, or will soon receive a PhD. Applications sent prior to the dissertation defense date are welcome. If you are interested in using single-particle cryo-EM and in situ (cellular) EM to elucidate cellular mechanisms, submit the following information to [email protected]mailto:[email protected]: 1) Your CV (including your publication list); 2) Cover letter (less than 2 pages) describing: (i) your previous work - focusing on your most interesting finding(s), (ii) your scientific interests, (iii) which aspects of translation regulation are of interest to you. 3) Email addresses of three references. Selected publications (accessible via the provided links): • Carbone CE, Demo G, Madireddy R, Svidritskiy E, Korostelev AA. “ArfB can displace mRNA to rescue stalled ribosomeshttps://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-020-19370-z&data=04%7C01%7CAnna.Loveland%40umassmed.edu%7Ce628d89d1f79409b644108d88804e705%7Cee9155fe2da34378a6c44405faf57b2e%7C0%7C0%7C637408902048319137%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=3h1xTVVhDutkCMiVWyFwLup5IzVlHzNwJPifARLl0L4%3D&reserved=0”. Nature Commun. 2020. • Loveland AB, Demo G, Korostelev AA. “Cryo-EM of elongating ribosome with EF-Tu GTP elucidates tRNA proofreadinghttps://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41586-020-2447-x&data=04%7C01%7CAnna.Loveland%40umassmed.edu%7Ce628d89d1f79409b644108d88804e705%7Cee9155fe2da34378a6c44405faf57b2e%7C0%7C0%7C637408902048319137%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=OwJ9MZRWpN4ZQofCsUXlD1%2Bm3dJZdg8Kj736iJ0xVUA%3D&reserved=0”. Nature. 2020. • Loveland AB, Svidritskiy E, Susorov D, Lee S, Park A, Demo G, Gao FB, Korostelev AA. “Ribosome inhibition by C9ORF72-ALS/FTD-associated poly-PR and poly-GR proteins revealed by cryo-EMfile:///C:/Users/PowersA/Dropbox%20(UMass%20Medical%20School)/Korostelev/Job...”. bioRxiv. 2020. • Susorov D, Egri S, Korostelev AA. "Termi-Luc: a versatile assay to monitor full-protein release from ribosomeshttps://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Frnajournal.cshlp.org%2Fcontent%2Fearly%2F2020%2F08%2F14%2Frna.076588.120.abstract&data=04%7C01%7CAnna.Loveland%40umassmed.edu%7Ce628d89d1f79409b644108d88804e705%7Cee9155fe2da34378a6c44405faf57b2e%7C0%7C0%7C637408902048329132%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=NI6Q8kXCtjc84z4aVgEGEsQOEoG08wCRMUqQl1m9sjA%3D&reserved=0”. RNA. 2020. • Svidritskiy E, Demo G, Loveland AB, Xu C, Korostelev AA. “Extensive ribosome and RF2 rearrangements during translation terminationhttps://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Felifesciences.org%2Farticles%2F46850&data=04%7C01%7CAnna.Loveland%40umassmed.edu%7Ce628d89d1f79409b644108d88804e705%7Cee9155fe2da34378a6c44405faf57b2e%7C0%7C0%7C637408902048329132%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=LPp6orK3diN10%2BeFTWzlVmPeXj%2FoHrh3t239XdVvfmk%3D&reserved=0”. eLife. 2019. • Loveland AB, Demo G, Grigorieff N, Korostelev AA. “Ensemble cryo-EM elucidates the mechanism of translation fidelityhttps://nam10.safelinks.protection.outlook.com/?url=http%3A%2F%2Fem.rdcu.be%2Fwf%2Fclick%3Fupn%3DKP7O1RED-2BlD0F9LDqGVeSIKXTPnBku1A9OWTgBG4xjQ-3D_rJ0Q-2F8zH14bc-2FDhyIYgAaaIoDtN-2BMVA8uQ8ik0weaaWU8PS-2BLdbyuwTFcQze4H7H3NsgdQgtZP22s4d-2F7Ah5F9SEpNUyeYFo-2BwrMeGTv8bX708iwdYFE7myt3Zo8qQ0eqg1fJusX-2BXV1bNBbSZvYswm3izFmUn2nSTQNeuX5exHYwsMvNwwmnHT-2F6JYMZF6tiuin7sd3OMFe1MjOiM4u2ETfX1BuxYea4ybObPoymQQ-3D&data=04%7C01%7CAnna.Loveland%40umassmed.edu%7Ce628d89d1f79409b644108d88804e705%7Cee9155fe2da34378a6c44405faf57b2e%7C0%7C0%7C637408902048339128%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=r7bwGDNHCxLlCJ1tRaM3zmCa1LRTxG5Fk8gEaSSbhiU%3D&reserved=0”. Nature. 2017. • Demo G, Rasouly A, Vasilyev N, Svetlov V, Loveland AB, Diaz-Avalos R, Grigorieff N, Nudler E, Korostelev AA. “Structure of RNA polymerase bound to ribosomal 30S subunithttps://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Felifesciences.org%2Farticles%2F28560&data=04%7C01%7CAnna.Loveland%40umassmed.edu%7Ce628d89d1f79409b644108d88804e705%7Cee9155fe2da34378a6c44405faf57b2e%7C0%7C0%7C637408902048339128%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=3LZ0TgqtAe%2BqXUq%2FSqL1rSww%2Bw4sfDttOS1sWuewSvU%3D&reserved=0”. eLife. 2017. • Abeyrathne PD, Koh CS, Grant T, Grigorieff N, Korostelev AA. “Ensemble cryo-EM uncovers inchworm-like translocation of a viral IRES through the ribosomehttps://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles%2FPMC4896748%2F&data=04%7C01%7CAnna.Loveland%40umassmed.edu%7Ce628d89d1f79409b644108d88804e705%7Cee9155fe2da34378a6c44405faf57b2e%7C0%7C0%7C637408902048349116%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=12IYwZEXPIVVGJx4PJFKq4PoNUa0Pze%2BKgi8JIE8KhQ%3D&reserved=0”. eLife. 2016. • Loveland AB, Bah E, Madireddy R, Zhang Y, Brilot AF, Grigorieff N, Korostelev AA. “Ribosome•RelA structures reveal the mechanism of stringent response activationhttps://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Felifesciences.org%2Farticles%2F17029&data=04%7C01%7CAnna.Loveland%40umassmed.edu%7Ce628d89d1f79409b644108d88804e705%7Cee9155fe2da34378a6c44405faf57b2e%7C0%7C0%7C637408902048349116%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=gKYMi0V8%2ByFj1C7Rv7PFK26N%2FvwsPfVjeqLYVDnwxW4%3D&reserved=0”. eLife. 2016. https://twitter.com/korostelevlabhttps://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Ftwitter.com%2Fkorostelevlab&data=04%7C01%7CAnna.Loveland%40umassmed.edu%7Ce628d89d1f79409b644108d88804e705%7Cee9155fe2da34378a6c44405faf57b2e%7C0%7C0%7C637408902048359112%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=kc3cSbAmCx2YDw7jcCAXehOznVbKfibSscFxR3B0UNg%3D&reserved=0 --- Anna Loveland, Ph.D. [email protected] 617 610 4321 (cell) Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender immediately and destroy or permanently delete all copies of the original message.