Hi Laurie, as others suggested please try the same refinement using the latest version of Phenix. If that does not help please send me all inputs off list and I will investigate. Pavel On 3/18/22 13:11, Mark Saper wrote:

Laurie,

Really bizarre.  The experts will have more info, I hope.  When you model the Tyr in the posiive density, is the geometry satisfactory (including main-chain)?  If so, then you might try turning off real space refinement and see what happens.  Check the occupancies of the Tyr also.

-Mark _____________________ *Mark A. Saper, Ph.D.* Associate Professor Emeritus of Biological Chemistry University of Michigan Medical School [email protected] |  +1 734 276 6505

Powered by *Mailbutlerhttps://www.mailbutler.io/?utm_source=watermark&utm_medium=email&utm_campaign=watermark-variant-primary*, the email extension that does it all

_______________________________________________ phenixbb mailing list [email protected] http://phenix-online.org/mailman/listinfo/phenixbb Unsubscribe:[email protected]