Elwood Can you send me the model? Cheers Nigel NB. Any files sent to me will be held in strictest confidence. On Mon, Aug 11, 2014 at 10:28 AM, Mullins, Elwood A. < [email protected]> wrote:

Hello all,

I'm having trouble refining a protein-DNA complex in which one of the DNA strands has a mixture of two different nucleotides at one position. Both are non-standard and require CIF files. I tried assigning each nucleotide the same chain identifier and the same residue number but unique alternate location identifiers. This didn't work. Phenix refined the model but only linked one of the nucleotides with the rest of the chain, pushing the 3' OH and 5' phosphate out of the density for the other nucleotide. Coot won't even open the model output by Phenix. I assume there's some way to do this.

Thanks in advance,

Elwood

Elwood A. Mullins, Ph.D. <> [email protected] <> tel 615.936.5234 <> fax 615.343.6707

_______________________________________________ phenixbb mailing list [email protected] http://phenix-online.org/mailman/listinfo/phenixbb

-- Nigel W. Moriarty Building 64R0246B, Physical Biosciences Division Lawrence Berkeley National Laboratory Berkeley, CA 94720-8235 Phone : 510-486-5709 Email : [email protected] Fax : 510-486-5909 Web : CCI.LBL.gov