How can I add another chain to my PDB by Coot
Dear All, By Phenix refine I got a PDB (Only one chain) and mtz output files. After analysis by Coot I find I need to add a protein fragment so that a large blob will disappear. I know I use Baton to construct the protein fragment. Will you please tell me how can I input the sequence file of the protein fragment so that I can refine the protein fragment by Coot? I am looking forward to getting your reply. Regards. Dialing
On 14/11/11 04:19, Dialing Pretty wrote:
Dear All,
By Phenix refine I got a PDB (Only one chain) and mtz output files. After analysis by Coot I find I need to add a protein fragment so that a large blob will disappear. I know I use Baton to construct the protein fragment.
Will you please tell me how can I input the sequence file of the protein fragment so that I can refine the protein fragment by Coot?
There are a number of ways to fit a loop/blob - the devil's in the detail. Perhaps: Calculate -> Fit Loop Extensions -> Modelling -> DBLoop... But to answer your question: You assign the sequence like this: Extensions -> Dock Sequence -> Associate Sequence... Paul.
participants (2)
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Dialing Pretty -
Paul Emsley