Hi Bryan, I haven't tried your approach, but my guess would be that the correct sequence file will be the better way to go... The side chains are fitted after building main-chain so the sequence is not going to affect the main-chain build in any direct way (though the sequence will help the wizard in identifying and correcting places where loops are built with the wrong number of residues). -Tom T At 01:32 PM 11/8/2007, you wrote:

i was thinking about giving phenix.autobuild a poly-Ser/Gly,Ala-intact (or other incomplete variant) _sequence_file_, so it doesn't inter alia put arginine into strands... though the solvent content will be imprecise..

has anyone noticed if such a trick was more/less favorable to the (proper) complete sequence approach? any pertinent detail or criticisms appreciated.

-bryan _______________________________________________ phenixbb mailing list [email protected] http://www.phenix-online.org/mailman/listinfo/phenixbb

Thomas C. Terwilliger Mail Stop M888 Los Alamos National Laboratory Los Alamos, NM 87545 Tel: 505-667-0072 email: [email protected] Fax: 505-665-3024 SOLVE web site: http://solve.lanl.gov PHENIX web site: http:www.phenix-online.org ISFI Integrated Center for Structure and Function Innovation web site: http://techcenter.mbi.ucla.edu TB Structural Genomics Consortium web site: http://www.doe-mbi.ucla.edu/TB