Subject: no kidding - we need small crystals!
Hi everybody, Any suggestions regarding how to generate small!! crystals? Yes, in this case that is what we want While we can generate crystals of size 100-500 microns, this time we something in the range of .5-5 microns. Any ideas and suggestions would be greatly appreciated, Cheers Jens
test varying concentrations of glycerol. Tim On Jul 5, 2012, at 7:55 AM, jens j birktoft wrote:
Hi everybody,
Any suggestions regarding how to generate small!! crystals? Yes, in this case that is what we want While we can generate crystals of size 100-500 microns, this time we something in the range of .5-5 microns. Any ideas and suggestions would be greatly appreciated,
Cheers
Jens
_______________________________________________ phenixbb mailing list [email protected] http://phenix-online.org/mailman/listinfo/phenixbb
Dear Jens,
It depends on the crystallization conditions. I am not sure from your
message whether you are looking for small crystals from any protein
(like lysozyme) or of a specific protein. If it is a specific protein
and if it crystallized in PEG conditions,you can increase the
concentration of PEG which might give you shower crystals of very
small size.
Jobi
On Thu, Jul 5, 2012 at 7:55 AM, jens j birktoft
Hi everybody,
Any suggestions regarding how to generate small!! crystals? Yes, in this case that is what we want While we can generate crystals of size 100-500 microns, this time we something in the range of .5-5 microns. Any ideas and suggestions would be greatly appreciated,
Cheers
Jens
_______________________________________________ phenixbb mailing list [email protected] http://phenix-online.org/mailman/listinfo/phenixbb
Try seeding so that you get 1000 crystals per drop for example. You know it's a pain to mount .5 micron crystals or do you want to suck them into a capillary ? You could also grind your big crystals and see if they still diffract. Jürgen On Jul 4, 2012, at 7:55 PM, jens j birktoft wrote: Hi everybody, Any suggestions regarding how to generate small!! crystals? Yes, in this case that is what we want While we can generate crystals of size 100-500 microns, this time we something in the range of .5-5 microns. Any ideas and suggestions would be greatly appreciated, Cheers Jens _______________________________________________ phenixbb mailing list [email protected]mailto:[email protected] http://phenix-online.org/mailman/listinfo/phenixbb ...................... Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu
Smash up big Crystals?
There was a guy working on powder diffraction of protein samples (Lysozyme,
natch) in Birkbeck whilst I was doing my PhD and we all groaned when he
grew enormous crystals and then ground them up to collect powder data.
Dave.
On Jul 5, 2012 12:56 AM, "jens j birktoft"
Hi everybody,
Any suggestions regarding how to generate small!! crystals? Yes, in this case that is what we want While we can generate crystals of size 100-500 microns, this time we something in the range of .5-5 microns. Any ideas and suggestions would be greatly appreciated,
Cheers
Jens
_______________________________________________ phenixbb mailing list [email protected] http://phenix-online.org/mailman/listinfo/phenixbb
Hi Jens, aren't microcrystals of the size you describe exactly what the free electron laser people need for their experiments? Not knowing of any specific reference I would search for what these guys do to make tiny crystals. Cheers, Wulf Am 05.07.2012 01:55, schrieb jens j birktoft:
Hi everybody,
Any suggestions regarding how to generate small!! crystals? Yes, in this case that is what we want While we can generate crystals of size 100-500 microns, this time we something in the range of .5-5 microns. Any ideas and suggestions would be greatly appreciated,
Cheers
Jens
_______________________________________________ phenixbb mailing list [email protected] http://phenix-online.org/mailman/listinfo/phenixbb
Hello, Definitely try microseeding: take a big crystal, put it in eppendorf with mother liquor ( the precipitant should be at a concentration higher than what you use to build the big crystals) and crash it by vortexing (put a small piece of plastic in the tube). If you use the vapor diffusion technique take a µl of this solution and mix it with your protein solution on a cover slip just as if you set a normal drop, and then seal the well. Normally youll get a lot of tiny crystals after day or two. Good luck Filip On 2012-07-05 01:55, jens j birktoft wrote:
Hi everybody,
Any suggestions regarding how to generate small!! crystals? Yes, in this case that is what we want While we can generate crystals of size 100-500 microns, this time we something in the range of .5-5 microns. Any ideas and suggestions would be greatly appreciated,
Cheers
Jens
participants (7)
-
Bosch, Juergen -
David Briggs -
Filip YABUKARSKI -
jens j birktoft -
Jobichen Chacko -
Timothy Springer -
Wulf Blankenfeldt