Hi Valerie, The functionality you are mentioning is obsolete for some time now. Therefore I would recommend updating to recent Phenix release or nightly, especially for real-space refine. If you already have restraints for your ligand, they should be sufficient. If not, you can make them using eLBOW. There is a video tutorial about how to find and change parameters in phenix.refine (real-space is very similar): https://www.youtube.com/watch?v=qB8W_6yuw5k&t=2s Generally, in Phil notation '*' marks selected options. Please get back to us if you have more questions. Best regards, Oleg Sobolev. On Fri, Mar 27, 2020 at 5:42 AM Valerie Biou wrote:

Hello

I am trying to impose restraints on my lipid ligand during real space refinement. I found the command

refinement.geometry_restraints.edits.scale_restraints { atom_selection = "resname LLP" scale = 2.5 apply_to = *bond *angle dihedral chirality

I have 2 questions 1) can those lines be called from the GUI and where? if not, how can I insert them in a previous command file? 2) what do the * stand for? is it to activate or cancel the selection of an item? sorry if those questions have been asked before, I can't find the command to browse the bb archive.

All the best,

Valérie

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