Yes, Phaser computes the structure factors using the supplied B-factors. All that really matters is the relative B-factors between different atoms, because the structure factors are normalized before they are used in molecular replacement calculations. Actually, B-factor adjustment is one of the useful things that can be done by the Sculptor program -- Gabor has found that increasing the B-factors of poorly-conserved and highly-exposed regions of the structure can increase the signal in molecular replacement searches. Best wishes, Randy Read On 18 Nov 2010, at 19:59, Bryan Lepore wrote:

does (or can) phaser use the search probe B-factors in any way?

-Bryan _______________________________________________ phenixbb mailing list [email protected] http://phenix-online.org/mailman/listinfo/phenixbb

------ Randy J. Read Department of Haematology, University of Cambridge Cambridge Institute for Medical Research Tel: + 44 1223 336500 Wellcome Trust/MRC Building Fax: + 44 1223 336827 Hills Road E-mail: [email protected] Cambridge CB2 0XY, U.K. www-structmed.cimr.cam.ac.uk