Hello Dmitry, Depending on the resolution of the map of the whole complex, you might need to apply secondary structure restraints or reference model restraints (to prevent the atomic models from deviating too much from their starting coordinates), or you might even need to only do rigid-body refinement. Without knowing the resolution range of this map, it is difficult to advise on a strategy. Cheers, Guillaume On 24 Oct 2024, at 13:28, Dmitry Semchonok mailto:[email protected]> wrote: Dear Colleagues, We're encountering an issue with composite model refinement and would appreciate your advice. Our scenario: 1. We built two separate models based on two subtracted maps of a single complex. 2. We have a lower-resolution map of the full complex. 3. We merged the two models into the full complex and refined it against the full map. The problem: When refining the merged model against the full map using real-space refinement in Phenix, the clashscore progressively worsens. Has anyone experienced similar issues, or can you offer suggestions on addressing this problem? Thank you for your insights. Best regards, Dmitry VARNING: Klicka inte på länkar och öppna inte bilagor om du inte känner igen avsändaren och vet att innehållet är säkert. CAUTION: Do not click on links or open attachments unless you recognise the sender and know the content is safe. _______________________________________________ phenixbb mailing list -- [email protected]mailto:[email protected] To unsubscribe send an email to [email protected]mailto:[email protected] Unsubscribe: phenixbb-leave@%(host_name)s När du har kontakt med oss på Uppsala universitet med e-post så innebär det att vi behandlar dina personuppgifter. För att läsa mer om hur vi gör det kan du läsa här: http://www.uu.se/om-uu/dataskydd-personuppgifter/ E-mailing Uppsala University means that we will process your personal data. For more information on how this is performed, please read here: http://www.uu.se/en/about-uu/data-protection-policy