helical structure - Real space refinement
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Dear colleagues, Is there any specific procedure for Real space refinement in Phenix, having a map and model when working with helical structure? Many thanks Kind regard, Dmitry
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Hi Dmitry, if your model is composed of chains related by some rotation/translation (does not matter what symmetry), you can ask Phenix real-space refinement to identify and use that symmetry as "NCS" constraints (yes, NCS is a legacy term from Xtallography!). Aprart from this there isn't anything particular.. Alternatively, you can manually specify the master and symmetry related chains when setting up refinement job. Pavel On 2/1/24 02:48, Dmitry Semchonok wrote:
Dear colleagues,
Is there any specific procedure for Real space refinement in Phenix, having a map and model when working with helical structure?
Many thanks
Kind regard, Dmitry
participants (2)
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Dmitry Semchonok
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Pavel Afonine