[phenixbb] Refining with NCS

Francis E Reyes Francis.Reyes at Colorado.EDU
Mon Aug 25 12:37:53 PDT 2008


Thanks for the reply Pavel

However, with regard to the NCS maps issue, which should I be building  
to?Does the 2fofoc that phenix puts out adjusted for ncs and use that?

Thanks

FR

On Aug 25, 2008, at 11:59 AM, Pavel Afonine wrote:

> Hi Francis,
>
>> Also if anyone has a paper/protocol for NCS refinement, I'd love to
>> check it out (to figure out which map to fit to).
>
> Just something to keep in mind... I always prefer to run two  
> refinements
> in parallel with and without using NCS. It's resolution (and
> data-to-parameters ratio) dependent. I've seen a  number of cases  
> where
> using the NCS is very beneficial and similar number of cases where  
> it's
> better to not use NCS (mostly at resolutions ~2-2.5A).
>
> I would also check manually the NCS groups that are found by
> phenix.refine automatically. I have some cases where automatic  
> detection
> did not yield a good selection.
>
> Pavel.
>
> _______________________________________________
> phenixbb mailing list
> phenixbb at phenix-online.org
> http://www.phenix-online.org/mailman/listinfo/phenixbb

---------------------------------------------
Francis Reyes M.Sc.
215 UCB
University of Colorado at Boulder

gpg --keyserver pgp.mit.edu --recv-keys 67BA8D5D

8AE2 F2F4 90F7 9640 28BC  686F 78FD 6669 67BA 8D5D




More information about the phenixbb mailing list