[phenixbb] poor phasing

vennila Natesan vennilakn at gmail.com
Mon Apr 27 05:30:48 PDT 2009


Dear Tom

Thankyou for your kind reply.I checked my data witn phenix.xtriage
and there are good anomalous signal (>0.05)in all the resolution shells.
Also the systematic absences show it is P43212.Still i couldnt
solve using phenix.Where is the thing going wrong?
Xtriage results also showed no twinning.


My previous message:
I am using phenix1.4-4 version. In autosol, got the score of 10.24 for one
iodine soaked data.
FOM:0.27 and only 54 residues ( that too wrongly) were built out of
129.(CC=0.4), R=0.49 and Rfree=0.574.
Regrading the data quality, Rsym:0.0448, Ranom:0.055, Resln:25.0-2.6A
Spacegroup is P43212, completeness:99.4%, Multiplicity:11.7, anomalos
signal is 1.52 at lowest resoln and higher in high resolution shells.

What might be reason for not  able to solve this structure?
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