[phenixbb] poor phasing
PDAdams at lbl.gov
Mon Apr 27 08:25:30 PDT 2009
As Tom suggests you may need to try P41212 - your systematic absences
can't distinguish between this and P43212.
Alternatively download the latest nightly build of Phenix and try that:
On Apr 27, 2009, at 5:30 AM, vennila Natesan wrote:
> Dear Tom
> Thankyou for your kind reply.I checked my data witn phenix.xtriage
> and there are good anomalous signal (>0.05)in all the resolution
> Also the systematic absences show it is P43212.Still i couldnt
> solve using phenix.Where is the thing going wrong?
> Xtriage results also showed no twinning.
> My previous message:
> I am using phenix1.4-4 version. In autosol, got the score of 10.24
> for one iodine soaked data.
> FOM:0.27 and only 54 residues ( that too wrongly) were built out of
> 129.(CC=0.4), R=0.49 and Rfree=0.574.
> Regrading the data quality, Rsym:0.0448, Ranom:0.055, Resln:25.0-2.6A
> Spacegroup is P43212, completeness:99.4%, Multiplicity:11.7, anomalos
> signal is 1.52 at lowest resoln and higher in high resolution shells.
> What might be reason for not able to solve this structure?
> phenixbb mailing list
> phenixbb at phenix-online.org
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