[phenixbb] Finding correct Se sites with autosol

YS. Chung chungys at univmail.cis.mcmaster.ca
Thu Aug 27 07:58:48 PDT 2009


Dear all,

I have a 3 angstrom structure which I am trying to solve with MAD or
SAD. The structure was previously solved with molecular replacement but
refinement could not continue due to poor phasing at the N-terminal
domain of the protein. I have run solve and autosol which are able to
find the Se sites. However, Se sites are not anywhere near the
methionines when I superimpose the ha.pdb and the pdb file that I
obtain from a new run of molecular replacement. The
exptl_fobs_phases_freeR_flags.mtz and the overall_best.pdb do not show
any connected densities. I have also tried autobuild with the solutions
from AutoSol, but the model that it builds in is incorrect. Any
suggestions would be appreciated. 

Thank you,
Yu Seon Chung



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