[phenixbb] Can Phenix do MR with medium/low res EM models?

Edward A. Berry BerryE at upstate.edu
Thu Mar 19 13:35:35 PDT 2009

Hi Gino,
Are your EM models electron density instead of coordinates?
this site outlines a procedure
for tranferring the map to a arge unit cell (i.e. lots of space around the model,
so in your case really lareg!), calculating SF's, and using them as
MR model in phaser:

Gino Cingolani wrote:
> Hi Phenix community,
> I was wondering if Phenix can handle MR starting from medium/low res EM
> models.
> I have a very large oligomeric protein (2.4MDa) with lots of NCS and >450
> Se-Mets.
> In principle, I could use low res MR phases from the "replaced" model to
> locate Se-sites and 
> then NCS-averaging to locate more sites and improve my phases.
> Any suggestions how to get Phenix to use an EM model?
> Has anybody done it successfully using Phenix?
> Thanks in advance,
> Gino
> ***********************************************************
> Gino Cingolani, Ph.D.
> Assistant Professor
> Dept. of Biochemistry and Molecular Biology
> SUNY Upstate Medical University
> 750 E. Adams Street, Syracuse, NY, 13210
> Tel.   (315) 464 8744
> Fax.  (315) 464 8750
> Email:  cingolag at upstate.edu
> ***********************************************************
> "Nati non foste per viver come bruti,  ma per seguir virtute e conoscenza"
> ("You were not born to live like brutes, but to follow virtue and
> knowledge")
> Dante, The Divine Comedy (Inferno,  XXVI, vv. 119-120)
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