[phenixbb] Double stranded DNA restraints?

Rajagopalan, Senapathy SRajagopalan at tmhs.org
Fri Apr 2 12:09:31 PDT 2010

Hi Everyone,

Is there a way to force phenix (autobuild) into building a contiguous double stranded DNA. Currently, when I am solving a protein-DNA complex using SAD data:

1) its not recognizing that DNA needs to be double stranded, so it is fitting one of the strands correctly (at least part of it), but the other strand is in the protein density region.

2) It breaks down each of the strand. So it is not fitting as a contiguous 30 mer but instead a 10 mer, two 6 mers and a 8 mer.


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