[phenixbb] Phaser SAD module - resolution question

Peter Grey petgxray at gmail.com
Fri Aug 13 01:55:52 PDT 2010

Dear Phenix community,

I am using Phaser SAD module with a poor MR model to locate the initial
heavy-atom sub-structure.
I have a 3A dataset but the anomalous signal is weak at the higher
resolution shells.
I would like to ask for your advice regarding the resolution I should use in
I thought that for the sake of just locating the sites I should use
relatively low resolution (let's say 3.5 or even 4A) and then run Phaser a
second time.
In this second run I would use the highest-resolution and let Phaser only
refine the sites (located in the first run) during one cycle and calculate
the combined phases.

Thank you in advance for your suggestions and advice,

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