[phenixbb] is derivative data useful for looking for the missingpart of the model?

[Jacob Keller]

> Hi Fengyun,
>
> How have you determined whether you in fact have the full-length protein 
> in your crystals? It sounds like, from your statistics, that everything is 
> already modelled--it seems hard to believe that 80% of the whole protein 
> would give such good R values. Do others agree?
>
> You could run some of your crystals on a gel next to a sample which is 
> known to be full-length, and you could get the answer.
>
> Jacob
>
> *******************************************
> Jacob Pearson Keller
> Northwestern University
> Medical Scientist Training Program
> Dallos Laboratory
> F. Searle 1-240
> 2240 Campus Drive
> Evanston IL 60208
> lab: 847.491.2438
> cel: 773.608.9185
> email: j-keller2 at northwestern.edu
> *******************************************
>
> ----- Original Message ----- 
> From: <fn1 at rice.edu>
> To: <phenixbb at phenix-online.org>
> Sent: Tuesday, June 15, 2010 5:59 PM
> Subject: [phenixbb] is derivative data useful for looking for the 
> missingpart of the model?
>
>
>> Hi everyone,
>>
>> I got a partial model, consisting of 80% of the whole molecule, from  the 
>> molecular replacement. The native data reflects to 2.3 A, and now  the 
>> R/Rfree= 0.22/0.28. My problem is that the missing part of the  model is 
>> important for us, but there is not any density for this  region now.
>>
>> Meantime, we have several heavy atom derivative data. My question is 
>> that if refine the model against the derivative data, will the heavy 
>> atom appear in the difference map if existing? Any suggestions on how  to 
>> use the derivative data to locate the missing part of model?
>>
>> Thanks in advance!
>> Regards,
>> Fengyun
>>
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>