[phenixbb] AutoMR:Multi-subunit model

[Thomas C. Terwilliger]

Hi Saif,

If I understand you correctly, you can specify the search model with 8
polypeptides as one PDB file and all 8 sequences (separated by blank lines
or lines starting with ">" in one sequence file.  Also component 1 can be
all 8 polypeptides and copies=1.

All the best,
Tom T

 >> Hello,
>>
>> I have a membrane protein complex made up of 8 different polypeptides
>> present in
>> two copies each (16mer). The asymmetric unit is the octamer, as determined
>> from
>> previous crystallographic studies. To carry out molecular replacement with
>> the
>> phases from the published structure in AutoMR, should I specify the
>> coordinates
>> of the search model and its overall sequence identity as one file or
>> should I
>> break it down for each subunit ? Also, for the asymmetric unit contents,
>> should
>> I specify the sequence or mass of each subunit separately (as component 1,
>> 2 and
>> so on) keeping the number of copies fixed at 1 ? There is no NCS.
>>
>> Thanks.
>> --
>> S. Saif Hasan
>>
>> Graduate Student
>> Department of Biological Sciences
>> Hockmeyer Hall of Structural Biology
>> Purdue University
>> 240 S. Martin Jischke Drive
>> West Lafayette, IN 47907
>>
>>
>>
>>
>>
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