[phenixbb] automr/phaser : b-factors

[Randy Read]

Yes, Phaser computes the structure factors using the supplied B-factors.  All that really matters is the relative B-factors between different atoms, because the structure factors are normalized before they are used in molecular replacement calculations.

Actually, B-factor adjustment is one of the useful things that can be done by the Sculptor program -- Gabor has found that increasing the B-factors of poorly-conserved and highly-exposed regions of the structure can increase the signal in molecular replacement searches.

Best wishes,

Randy Read

On 18 Nov 2010, at 19:59, Bryan Lepore wrote:

> does (or can) phaser use the search probe B-factors in any way?
> 
> -Bryan
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> phenixbb at phenix-online.org
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------
Randy J. Read
Department of Haematology, University of Cambridge
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