[phenixbb] automr/phaser : b-factors
rjr27 at cam.ac.uk
Thu Nov 18 13:48:06 PST 2010
Yes, Phaser computes the structure factors using the supplied B-factors. All that really matters is the relative B-factors between different atoms, because the structure factors are normalized before they are used in molecular replacement calculations.
Actually, B-factor adjustment is one of the useful things that can be done by the Sculptor program -- Gabor has found that increasing the B-factors of poorly-conserved and highly-exposed regions of the structure can increase the signal in molecular replacement searches.
On 18 Nov 2010, at 19:59, Bryan Lepore wrote:
> does (or can) phaser use the search probe B-factors in any way?
> phenixbb mailing list
> phenixbb at phenix-online.org
Randy J. Read
Department of Haematology, University of Cambridge
Cambridge Institute for Medical Research Tel: + 44 1223 336500
Wellcome Trust/MRC Building Fax: + 44 1223 336827
Hills Road E-mail: rjr27 at cam.ac.uk
Cambridge CB2 0XY, U.K. www-structmed.cimr.cam.ac.uk
More information about the phenixbb