[phenixbb] MR problem
faisaldbg at gmail.com
Mon Aug 22 00:12:18 PDT 2011
I have a data of heterotrimer protein complex at 2.9A resolution.
One protein consists of two domains. I tried phaser as well as molrep which
gives a solution with only one domain.
Rotation function and translation function were found to be fine for these
I tried to find missing domain of the protein after fixing one of the domain
or the other partner proteins using whole part or various truncations of
I also tried to find and build missing domain using Rosetta with the
solutions of Molrep or Phaser as template.
But, there were no solutions, or the solutions are clashed with other domain
Furthermore, R and Rfree is 30 and 40, respectively, and I could not reduce
There was almost no electron density map in the empty space so that I could
not model manually.
Plz guide me ,how can i look for the missing domain in the protein.
LABORATORY OF STRUCTURAL BIOINFORMATICS
KOREA UNIVERSITY, SEOUL
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