[phenixbb] strategy suggestion ???
nechols at lbl.gov
Tue May 31 10:05:03 PDT 2011
On Mon, May 30, 2011 at 6:31 PM, ChenTiantian <chentiantian2010 at gmail.com>wrote:
> 1, Processing data with XDS (or HKL2000 for some of the datasets)
> 2,Molecule replacement with phaser.
> 3,Check and modify the structure residue by residue with COOT,
> 4,phenix.refine with the following command line:
Step 3 is probably redundant - you can usually go straight to refinement,
and afterwards the maps will be much clearer and easier to correct manually.
Add the rigid_body strategy if you're worried about the overall orientation
of domains, and fix_rotamers=True to correct individual residues.
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