[phenixbb] MR_ROSETTA

Wei Shi Wei.Shi.GR at dartmouth.edu
Fri Nov 18 09:07:12 PST 2011

Thank you, Nat! I will try phenix.sculptor at the same time!

From: phenixbb-bounces at phenix-online.org [phenixbb-bounces at phenix-online.org] on behalf of Nathaniel Echols [nechols at lbl.gov]
Sent: Friday, November 18, 2011 10:54 AM
To: PHENIX user mailing list
Subject: Re: [phenixbb] MR_ROSETTA

On Fri, Nov 18, 2011 at 7:50 AM, Wei Shi <Wei.Shi.GR at dartmouth.edu> wrote:
> The sequence identity of the search model to my protein is 33%. I haven't tried mr_rosetta using this search model without pre-refinement. I only used this search model directly to do molecular replacement using phenix, and no viable solutions are generated.

It may also help to run the model through phenix.sculptor, which will
modify it to more closely match the target protein (without doing
anything to the conformation).  Sometimes this is all that you need to
make MR successful - although it's never guaranteed to work at 33%

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